Question

Which of the following microorganisms is best suited as a cloning vector to introduce nematode specific genes in plants ?

Answer 1

Answer:

Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double-stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant, therefore, got itself protected from the parasite.

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