Which of the following statements is true for a refractive index detector in HPLC?
A.It is more sensitive than a UV detector
B. It can only be used for isocratic elutions
C. It does not respond to many solutes
D. none of above
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OPTION [C] is correct ✅✅✅
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OPTION [C] is correct ✅✅✅
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Answer:
In HPLC, a refractive index detector does not respond to many solute particles.
Therefore, the option (C) is correct.
Explanation:
Full form of HPLC is high performed liquid chromatography. HPLC is a modern analysis technique which is used in various fields such as biochemical, organic and inorganic compounds.
- HPLC gives perfect results in the separation, identification and quantification of the analyte to be analyzed.
- HPLC instrumentation involves pump, injector, detector, column, integrator and a display system.
- The separation occurs at the column.
Refractive index detector:
- In HPLC, refractive index detector is a universal detector because of its identification properties.
- Refractive index detector does not respond to many solute particles. Refractive index detector only those particles which have refractive index different from refractive index of solvent.
Ultraviolet (UV)detector is most commonly and simplest HPLC detector because of its greater sensitivity, linearity, versatility.
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