Question

which of the following techniques the DNA is lobed using complementary sequences and then analyzed using auto radiography?
a) western blotting
b) southern blotting
c) isopycnic centrifugation
d) northern blotting ​

Answer 1

Answer:

Southern blotting

Explanation:

Southern blotting is a technique used to detect DNA sequence of interest by using complementary DNA probe. The probe is labelled using radioactive isotopes and contains complementary sequence to the target DNA molecules. Once the probe hybridizes (forms base pairing) with desires DNA molecule in the sample, they can be detected using the radioactive capacity of probe. The blotted paper is analyzed via auto radiography to detect the location of probe-dna base pairing. Thus gene of interest can be isolated