which part doesnt take stain in chromosome
Answers
Chromosomal Staining Methods
I’ve listed a few of the ways this can be accomplished:
G-Banding – Chromosomes are stained with giesma stains. The appearance differs based on the treatment of chromosomes prior to staining.
Q-Banding – Chromosomes are stained with fluorescent dyes, quinacrine or quinacrine mustard. Q-Band staining is similar to G-banding in that the fluorescent regions represent the AT-rich regions of the chromosome.
R-Banding – Results from heat treatment in a phosphate buffer followed by staining with Giesma dyes.
C-Banding – Centromere staining that results from alkali treatment.
So how do you exactly identify chromosomal location based on banding patterns?
In studying disease and mutation, we follow a specific type of nomenclature to designate the regions that are of interest to us. Let’s take for instance something like the 22q11.2 deletion. What do all of these numbers and letters mean?
To quickly summarize, 22q11.2 deletion syndrome occurs from the deletion of a small piece of chromosome 22 at a location: q11.2
So now, when we add in karyotope information you might see something like the following:
46, XY, del(8)(q21)
When you break it down, it states the patient is male (XY) and has a deletion in the long arm (q) of chromosome 8 at region 2, band 1
Translocation nomenclature can get a little more confusing:
46, XX, t(3;12)(p12.1;p11)
This designates a female has a translocation between the short arms (p) of chromosomes 3 and 12 and region 1, band 2, sub band 1; and region 1 band 1 respectively.
An example of Down syndrome: 47, XX + 21 (Female has an extra chromosome 21)
An example of Klinefelter Syndrome: 47, XXY (Male with extra X chromosome)
Answer:
right side of chromosome