which part of cuscuta is made of apex
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Cuscuta root-like structure and shoot apex at 1st–7th day after germination were hand cut and fixed in 4% (w/v) paraformaldehyde in PEM buffer (50 mM PIPES, 5 mM EGTA, 5 mM MgSO4, pH 6.8) for 1 h. After washing in PEM, cell walls were digested by a cocktail of 3% (w/v) macerozyme R10 and 3% (w/v) cellulase R10 (Duchefa) in PEM at room temperature for 1.5 h
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