Which precautions should be kept in mind while preparing a temporary slide of an epidermal peel of a leaf ?
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1)The epidermal peel should be taken from a freshly-plucked leaf.
2)Take the epidermal layer from the lower surface of a leaf, as it has more stomata.
3)Always use a clean glass slide.
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4
Cut the peel of the leaf in proper size while avoiding its folding.
The peel should always be placed at the centre of the slide while holding the edges.
Overstraining and under-straining of the peel should be avoided.
Avoid using needle as it might damage the cells.
Use brush for easy handling.
Prevent the drying of the peel while using glycerine.
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