Question

which primer are used in annealing during amplification of gene?​

Answer 1

Answer: oligonucleotide primers

Explanation: At this temperature, the oligonucleotide primers can form stable associations (anneal) with the denatured target DNA and serve as primers for the DNA polymerase.

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Answer 2

In PCR, the mixture is allowed to cool ( 40°-60°C) that permits pairing of the primer to the complementary sequences in DNA, this step is called an annealing.

Reverse primers used in annealing during amplification of gene.

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