Which reaction is used for quantity of total cholesterol in blood plasma.
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Cholesterol analysis was first reported by Liebermann in 1885 followed by Burchard in 1889. In Liebermann-Burchard reaction, cholesterol forms a blue-gree dye from polymeric unsaturated carbohydrates in an acetic acid/acetic anhydride/concentrated sulfuric acid medium.
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The modified Abell-Kendall method is a standard reference method for the determination of total cholesterol for clinical use approved by NIST. It is a multi-step classical chemical method involving Liebermann-Burchard (L-B) reaction for colour development
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