Question

which reagent is used as a spraying agent
to detect amino acid on TLC Plates?
1) Bromocresol green
2) Ninhydrin
3)Fuchsin
4) Rhodamine B​

Answer 1

Explanation:

2-hydroxy-1-naphthaldehyde

A new spray reagent, 2-hydroxy-1-naphthaldehyde has been introduced for the detection of amino acids

Answer 2

Spraying agent  to detect amino acid on TLC Plates

Explanation:

Correct option: (2) Ninhydrin reagent

• To detect the presence of amino acids on developed TLC plates, Ninhydrin reagent can be used.
• Chemically, Ninhydrin reagent is: 2,2-dihydroxyindane-1,3-dione
• Function: The function of Ninhydrin reagent is to detect primary and secondary amines in a reaction mixture.
• On reaction of ninhydrin reagent with primary amines, intense blue color is obtained.
• With secondary amines, less intense brown-red color is seen.
• Reaction of amino acid with Ninhydrin agent gives dark purple colored product (Ruhemann's purple).
• TLC (Thin layer chromatography) plates are used for separation of constituents from a mixture and identification of analyte.