Which restriction enzyme is used in recombinant dna tech?
Answers
Recombinant DNA is the term applied to chimeric DNA molecules that are constructed in vitro, then propagated in a host cell or organism. The basic recombinant DNA consists of a vector and an insert (Figure 1). The vector is a replicon (see Replicon) capable of replicating in the cells of choice. It is endowed with a functional replication origin, usually carries a selectable marker, and typically has been engineered to accommodate inserts conveniently. Vectors are based on naturally occurring replicons, such as bacterial plasmids, viruses, or cellular chromosomes. Inserts can be of any sort – long or short segments of DNA, from natural or synthetic sources. The resulting recombinant DNAs are often referred to as clones, which is shorthand for chimeric DNAs that are isolated in cellular or viral clones; and the process of producing these recombinants is frequently called DNA cloning or gene cloning.