Question

Which restriction enzyme, makes single-stranded breaks four bases apart in the opposite strands of its target sequence so generating fragments with protruding 5' termini and hence, fragments with sticky or cohesive ends ? 5'-G 5'-AATTC-3' 3'-CTTAA-5' G-5'

Answer 1

Eco R I

5' --- GAATTC --- 3'

3' ----CTTAAG --- 5'

            ↓↓↓

5' --- G                   AATTC --- 3'

3' --- CTTAA                    G --- 5'