Question

which solution is used to find fine bacteria in gram stain

Answer 1

Grain staining

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1. The primary stain (crystal violet reagents for staining)

- Solution A

• 4 grams of crystal violet (certified 90 percent of the dye content)

• 40ml of ethanol (95percent vol/vol)

- Solution B

• 0.16 grams of ammonium oxalate,

• 120ml of distilled water,

Mix A and B so as to obtain crystal violet staining reagent and store for 24 hours.

2. Mordant (grams iodine)

• gram of iodine,

• grams of potassium iodide,

• 600ml of distilled water,

Using a mortar, iodine and potassium iodide are ground, while slowly adding water with continued grinding until all the iodine has completely dissolved. (Store this in an amber bottle)

3. Decolorizing agent

• Ethanol, 95 percent (vol/vol)

However, acetone or 1:1 acetone with ethanol,

• 100ml acetone

• 100ml ethanol

4. Counterstain (safranin)

- Working solution:
•
20ml of the stock solution (5 grams Safranin O and 200ml of 95 percent ethanol),

• 180ml of distilled water,