Which stain is used for straining stem tissues? Safranin Methylene Eosion Iodine
Answers
Explanation:
It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. Safranin (also Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red.
safranin staining
The safranin staining is the most widely used staining technique for cell differentiation, cell-based assays, and stem cell culture. The safranin stain is commonly used to quantify and identify the acidic proteoglycan and glycosaminoglycan in the cartilage tissues.
In Gram's staining, the safranin directly stains the bacteria that has been decolorized. With safranin staining, the gram-negative bacteria can be easily distinguished from gram-positive bacteria.
Hoechst stains - two types of fluorescent stains, 33258 and 33342, these are used to stain DNA in living cells. Iodine - used as a starch indicator. When in solution, starch and iodine turn a dark blue color.