Question

Which technique is used to find molar masses of proteins , polymers and other macromolecules and why?

Answer 1

Answer:

While there are many ways to determine the molar masses, or molecular weights, of macromolecules such as proteins, biopolymers and synthetic polymers, only one determines absolute molecular weights in solution, rapidly and effectively: Multi-Angle static Light Scattering (MALS).

Answer 2

║⊕ANSWER⊕║

• Osmotic pressure is preferred colligative property to determine molecular mass of macromolecules. -
• It can be measured with precision even in dilute solutions. - Variation in osmotic pressure is follows colligative law.
• As compared to other colligative properties, its magnitude is large even for very dilute solutions.
• The technique of osmotic pressure for determination of molar mass of solutes is particularly useful for biomolecules as they are generally not stable at high temperatures and polymers have poor solubility.
• In osmotic pressure method, pressure is measured at room temperature and instead of molality, molarity of the solution is used.
• It is preferred for the macromolecules like proteins as they are unstable at high temperature and polymers have poor solubility.
• Therefore, osmotic pressure method is preferred.