Question

Which type of restriction endonuclease cuts the DNA within the recognition site?
A.Type I
B. Type II
C. Type III
D. All of the these

Answer 1

The answer is option B.

Answer 2

Answer:

The correct answer is B. Type II.

Explanation:

Restriction endonuclease type II are the ones that cleave the DNA sequences at a specific site. This specific site is present in the recognition site only, whereas the other two types cleave DNA sequences randomly. These are the most commonly used RE for this reason only. However, they are also an important component in the restriction-modification systems i.e. defense mechanism of bacteria. RE type II requires Mg2+ for the catalysis.

#SPJ3