Biology, asked by scantishunma, 1 year ago

While doing PCR 'denaturation step is missed, How will be the process be affected?

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Answered by Manjula29

Denaturation is process in which double helical DNA unwinds at high temperature to produce single stranded DNA templates. If the denaturation step is missed, the single stranded template needed for annealing of primars will not be produced. Hence, PCR reaction will not proceed further.

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