Question

While performing "Identification of blood group" experiment . Satheesh and
Seetharam found that agglutination does not occur in anti - RhD serum after
adding blood sample. This denotes :
( )
A) Rh factor is negative
B) Rh factor is positive
C) Rh factor is neither positive nor negative D) Rh factor is slightly acidic​

Answer 1

Option A) Rh factor is negative

Points to know:

Identification of blood group -

-

There are antigen (A, B and D} present in surface of RBC in blood

There are antibodies { anti-A, anti-B and anti-D present in serum.

In blood group typing, we mix formed antibodies to blood of human. Then we see agglutination

(that is clumping)

Now, the antibodies with which blood is agglutinated, corresponds to the blood group of individual.

1. If agglutination occurs with anti-A, then blood group is A

2. If agglutination occurs with anti-B, then blood group is B

3. If agglutination occurs with both anti-A and anti-B blood group is AB

4. If agglutination doesn't occur with any, that is anti-A and anti-B, then blood group is O

Now, depending on agglutination with anti-D

If agglutination occurs - Rh+

If agglutination doesn't occur - Rh