Question

While preparing a temporary mount of cheek cells, teacher asked Rohit to pick up the stain. He had four bottles A, B, C and D containing Methylene blue, glycerine, distilled water, Canada balsam respectively. Which one should he pick?

A
B
C
D

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Answer 1

Answer:

Option A - Methyle Blue

Explanation:

Methyle blue, is a staining agent that is used in place of safrinin solution to stain the cell. Stains are usally used while observing the cell under a microscope as the cells are usally colourless and need some sort of colour to make it visible under a compound microscope.

Answer 2

He should pick a bottle containing Methylene blue which is- A

• The methylene blue is a stain which stains the negatively charged molecules present in the cell. Hence, it is used to stain animal cells because it binds to the DNA present in the nucleus of cell. This makes the nucleus to be easily visible and we can easily identify it under the microscope.
• The nucleus appears dark blue in colour while the rest part of the cell appears light blue in colour.
• As cheek cells are animal cells, hence, Rohit should pick bottle A containing methylene blue stain.