Question

While preparing a temporary mount of stomata four students used different stain which are given below
A acetocarmine
B. Methylene blue
C. Safranin
D. Iodine
Which student used the correct stain and why

Answer 1

Safranin is the right answer

Answer 2

In the preparation of temporary stomata and in peeling leaf, the stain used is SAFRANIN. So, the student who is using safranin is possessing correct stain.

The safranin is red in colour which can stain the plant materials very easily. The plant materials would be lignin and suberin.

Since, the stain of the safranin is red, the tiny spores of stomata is very clearly visible. The dye also leaves red colour on the nuclei of the cells which makes it easy during identification.