Biology, asked by nehadot20pe4fym, 1 year ago

While preparing a temporary stained Mount of a leaf epidermal peel to show stomata what is the correct procedure for
a)removal of extra stain b)mounting of the material

Answers

Answered by babai13
6
Procedure:

1. Remove a healthy leaf from the potted plant.

2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.

3. Put a few drops of safranin stain in a watch glass.

4. After 2-3 minutes take out the peel and place it on a clean glass slide.

5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.

6. Remove the excess stain and glycerin with the help of blotting paper.

7. Observe the slide under the low-power and high-power magnifications of the compound microscope.

hope this helps
these are only procedures
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Answered by kavya005
7
1.
Use blotting paper to remove the extra fluid or stain.
2.
Firstly, place a drop of clear water on the slide. Then, take the required sample with the help of a paintbrush and transfer it to the slide. Now, cover the sample with the help.of a mounting needle.
How to mount?
Keep the cover slip at an angle and hold it with the help of a mounting needle. Slowly, take the needle away from the cover slip and let it fall on the sample. This is the best way to mount samples without the presence of air bubbles.

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