Question

while preparing temporary mount of stomata a.acetocarmine b.methylene blue c.safranin d.iodine which is correct stain and why

Answer 1

The correct answer is the option C - Safranin.

Safranin is used as the stain while preparing a temporary mount of the stomata in a leaf peel. The leaf peel is carefully peeled off and left in the safranin dye for 2 - 3 minutes, after which it is mounted using glycerin.

Safranin is a biological dye that is used as a counterstain in many cytological and histological experiments. The dye leaves a red colour on the nuclei in the cells, which makes it easy to identify.

Answer 2

Safranin stain is used in the preparation of temporary mount of stomata. Safranin solution is used in the preparation of slide.

Take few drops of safranin in a watch glass. Dip epidermis in it for 30 sec to stain the peel of stomata.  It is used as a staining dye. It gives color to leaves, so easily seen under a microscope.

Hope this will prove helpful for you.