Question

WHO USED A CELL FREE SYSTEM FOR PROTEIN SYNTHESIS

Answer 1

Answer:

Ribosome

Explanation:

Ribosomes are found in every major cell type and are the site of protein synthesis.

Answer 2

Marshall Nirenberg and Heinrich J. Matthaei were the first to use cell-free protein synthesis.

• At the National Institutes of Health, they defined the first codon in 1961.
• Transcribing a poly-uracil RNA sequence in a cell-free system, they discovered that their synthetic polypeptide consisted only of phenylalanine.
• Cell-free reactions are typically composed of a cell extract, an energy source, amino acids, cofactors like magnesium, and the DNA containing desired genes.
• Energy is usually supplied from pyruvate, acetyl phosphate, and creatine phosphate, along with amino acids, to a separate mixture which is then added to the extract to initiate the reaction.

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