Biology, asked by bioexpress, 1 month ago

wht are the cloning sites_??​

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Answered by ՏɑɾíƙɑՏօƖɑղƙí
0

Answer:

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wht are the cloning sites_??

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Cloning sites : In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition site within the vector will generate several fragments, which will complicate gene cloning. The gene of interest is inserted at restriction enzyme site located in any one of the antibiotic resistance genes or selectable marker genes, such that, recombinant vector will lose antibiotic resistance due to insertion of foreign DNA to one selectable marker, but can still be selected out from non-recombinant ones by presence of intact antibiotic resistance for another selectable marker. A cluster of unique restriction enzyme sites in close proximity in a plasmid forms a region called multiple cloning site (MCS).

Answered by jahnu12keerthika
0
A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid.
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