wht is audiography and its purpose_?
Answers
Answer:
wht is audiography and its purpose_?
It is a method allowing the detection/localisation of radioactive isotope within a biological sample.
Purpose of autoradiography :
To demonstrate the distribution of a radiolabled compound within an organism or a cell or to localise the site of incorporation of this compound into the cells or tissues.
Some molecular diagnostic techniques such as RFLP make use of hybridisation probes to detect mutated genes causing disease.
A single stranded DNA or RNA tagged with a radioactive molecules called probe allowed to hybridise to its complementary DNA in a clone of cells followed by detection causing autoradiography. the clone having the mutated gene will not appear on the photographic film, because the probe will not have complementarity with the mutated gene.
To detect hybridisation of the probe to its target sequence, the probe is tagged (or labelled) with a molecular marker of either radioactive (more recently fluorescent molecule). Commonly used marker are p32 incorporated into the phosphodiester bond in the probe DNA.
Answer:
Audiography ("writing sound") within Indian-style filmmaking, is the audio engineering performed by the sound department of a film or TV production; this includes sound recording, editing, mixing and sound design but excludes musical composition, songwriting and choreography
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