Question

wht is the strategy to prevent anti sense infestation_??​

Answer 1

Answer:

$\underline \mathscr \purple{Question \: : - }$

wht is the strategy to prevent anti sense infestation_??

$\underline \mathscr \purple{Answer \: : - }$

RNAi is a naturally occurring mechanism that leads to the "silencing" of genes. In consequence, the respective protein is no longer synthesized. In nature, this mechanism is used for the regulation of specific genes and is also applied as a defence against viruses. In research, this technique has been used for loss of function studies where a gene (responsible for parasitism) is specifically silenced. It takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to formation of dsRNA molecule formed by binding of complementary RNA (anti-sense RNA) molecule to original mRNA, thereby preventing translation of the original mRNA (silencing). The source of this complementary RNA could be from infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate.

Using Agrobacterium vectors, nematode specific genes (responsible for parasitism) were introduced into the host plant. The introduction of DNA was such that it produced both sense anti-sense RNA in the host cells forming dsRNA. These two RNA's being complementary to each other formed a dsRNA that was taken up by the parasitic nematode and initiated RNAi, thus silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA The transgenic plant therefore got itself protected from the parasite.