Biology, asked by rohitpawarjath, 2 months ago

why 0.1 N HCL used in estimation of haemoglobin

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Answered by pruthagauri

Answer:

Object: Quantitative estimation of hemoglobin by Sahli's (acid-hematin) method.

Principle: When blood is mixed with 0.1 N HCl (N/10 HCl), the Hb is degraded to give rise brown-colored acid-hematin. The intensity of color of acid-hematin is directly proportional to the concentration of Hb.

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why 0.1 N HCL used in estimation of haemoglobin​

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why 0.1 N HCL used in estimation of haemoglobin