Why 2m tris base is used to adjust ph of antibodies?
Answers
Sodium azide is used to arrest the growth of the yeast. It can be stored at room temperature as a 1 M solution. All the purification solutions contain the urea buffer (6 M urea, 100 mM Na2H2PO4, pH 8, 10 mM Tris, pH 8, 10 mM 2-mercaptoethanol). The stock 8 M urea solution is made the day before the purification and deionized by stirring overnight at 4° with AG501-X8(D) resin (Bio-Rad, Hercules, CA). The other stock solutions are stored at room temperature: 1 M Tris, pH 8, 1 M Na2H2PO4, pH 8 (pH is adjusted with NaOH), 5 M NaCl, 2 M imidazole, pH 8. The working solutions are prepared on the day of the purification and are degassed followed by filtration. The α-factor is dissolved in sterile water at a concentration of 1 mg/ml and stored in 1 ml aliquots at − 20°. We obtain α-factor from a peptide synthesis facility (Keck Facility, Yale University).