Science, asked by shivi2630, 11 months ago

Why 32 P is added at 5 end of dna in dna sequencing? best will b marked as brainliest​

Answers

Answered by kakkerisunil
0

★DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA.

★In Sanger sequencing, the target DNA is copied many times, making fragments of different lengths. Fluorescent “chain terminator” nucleotides mark the ends of the fragments and allow the sequence to be determined.

★Next-generation sequencing techniques are new, large-scale approaches that increase the speed and reduce the cost of DNA sequencing.

I think it will help you.....

★The same primer, template DNA, DNA polymerase, ddTTP, dNTP's (dATP, dTTP, dCTP, dGTP with one having a 32P label) are

incubated together

produces DNA

★Have the same 5' end due to same primer sequence

★Have the same 3' end due to the ddTTP inhibition

★Oligonucleotide fragment length will be DIFFERENT!


shivi2630: this is not wt i have asked
kakkerisunil: Sorry
kakkerisunil: I have edited the answer
kakkerisunil: I think you got the answer
shivi2630: can u explain this in ur own simplified language
kakkerisunil: I will explain you later
shivi2630: ok
kakkerisunil: Super line's
kakkerisunil: in profile
shivi2630: ty
Answered by anubha10
1

Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' dideoxynucleotides, and abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP).


shivi2630: not wt i asked
anubha10: sorry
Similar questions