Why 32 P is added at 5 end of dna in dna sequencing? best will b marked as brainliest
Answers
★DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA.
★In Sanger sequencing, the target DNA is copied many times, making fragments of different lengths. Fluorescent “chain terminator” nucleotides mark the ends of the fragments and allow the sequence to be determined.
★Next-generation sequencing techniques are new, large-scale approaches that increase the speed and reduce the cost of DNA sequencing.
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★The same primer, template DNA, DNA polymerase, ddTTP, dNTP's (dATP, dTTP, dCTP, dGTP with one having a 32P label) are
incubated together
produces DNA
★Have the same 5' end due to same primer sequence
★Have the same 3' end due to the ddTTP inhibition
★Oligonucleotide fragment length will be DIFFERENT!
Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' dideoxynucleotides, and abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP).