Why 4 to 6 week old arabdopsis thaliana plant leaf protoplast is used?
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Plants are 4–6 weeks old when the leaves are harvested and have not yet initiated flower bolts. Protoplasts are isolated from healthy green .
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The current standard Arabidopsis mesophyll protoplast isolation protocol is well-established, and has also been used in various modified forms for specific needs [4]. In this protocol, five-week-old plants are used for protoplast isolation.
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