Why 70 ethanol is used in dna extraction?
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DNA is washed with 70% ethanol to remove some (or ideally all) of the salt from the pellet. If water was used as the wash then DNA would dissolve again and if 100% ethanol was used the salt would not wash off because sodium salts are poorly soluble in ethanol. because precipitation in 100% ethanol cause removal of all water molecule from DNA and Complete Dehydration,which make them not soluble, So we give 70% wash to let it retain some water molecule when make it soluble.
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The main role of monovalent cations and ethanol is to eliminate the solvation shell that surrounds the DNA, thus allowing the DNA to precipitate in pellet form. Additionally, ethanol helps to promote DNA aggregation. Usually, about 70 percent of ethanol solution is used during the DNA washing steps
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