Biology, asked by madhuvanthani3889, 1 year ago

Why absorance intensity increases for denaturation of dna?

Answers

Answered by Ajay1291

The phenomenon of UV absorbanceincreasing as DNA is denatured is known as the hyperchromic shift. The purine and pyrimidine bases in DNA strongly absorb ultraviolet light. Double-stranded DNA absorbs less strongly than denatured DNA due to the stacking interactions between the bases.

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