Why are not all phenolics visualized in chromatography?
Answers
Answer:
1-Methods of analysis
TLC was performed with two different chromatographic systems. Thin layers were
prepared from microcrystalline cellulose and silica gel G. Standard solutions and samples were spotted on precoated plates of cellulose microcrystalline with fluorescent indicator and developed with mobile system of ethyl-acetate:formic acid:acetic acid:water (100:11:11:26, v/v/v/v). The other chromatographic system was silica gel GF254 as stationary and chloroform:ethyl-acetate:formic acid (5:4:1, v/v/v) as mobile phase. Substances were identified using UV detection at 254 nm. For visualization, plates were sprayed with FeCl3 (2% in ethanol).
Answer:
1-Methods of analysis
TLC was performed with two different chromatographic systems. Thin layers were
prepared from microcrystalline cellulose and silica gel G. Standard solutions and samples were spotted on precoated plates of cellulose microcrystalline with fluorescent indicator and developed with mobile system of ethyl-acetate:formic acid:acetic acid:water (100:11:11:26, v/v/v/v). The other chromatographic system was silica gel GF254 as stationary and chloroform:ethyl-acetate:formic acid (5:4:1, v/v/v) as mobile phase. Substances were identified using UV detection at 254 nm. For visualization, plates were sprayed with FeCl3 (2% in ethanol).