Chemistry, asked by princessy5056, 6 months ago

Why concentrated sample can not study in UV-vis and fluorescence spectroscopy? *​

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Answered by Angelpriya80
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Answer:

However, too concentrated a solution decreases the fluorescence intensity, as shown in Figure 3.22(a). Further increases in concentration induce change in the shape of the fluorescence spectrum because the fluorescence at shorter wavelengths is absorbed by other molecules of the same species (Figure 3.22(b)).

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