why did hersey and chase use radioactivity labelled phosphorours and sulphur only? would they have got same result if they use carbon and nitogen?
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Answer:
P32 S35 are Radioactive Isotopes .
Nitrogen not has Radioactive Isotope
N15 is heavy Isotope.
Explanation:
Use of 15N will be inappropriate because method of detection of 35p and 15N is different (32p being a radioactive isotope while 15N is not radioactive but is the heavier isotope of Nitrogen). Even if 15N was radioactive then its presence would have been detected both inside the cell (15N incorporated as nitrogenous base in DNA) as well as in the supernatant because 15N would also get incorporated in amino group of amino acids in proteins). Hence the use of 15N would not give any conclusive results.
- Refer Ncert class 12 Biology
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