Why DNA cannot pass through the cell membrane? How can the bacteria be made competent to take up a plasmid? Explain a method for introduction of alien DNA into a plant host cell. Name a pathogen that is used as a disarmed vector.
Answers
Answered by
0
DNA cannot pass through the cell membrane because it is there life
Answered by
0
Use of disarmed factor in plant host cell
Explanation:
- DNA has a sugar-phosphate foundation, which makes it hydrophilic in nature thus DNA can't go through the cell layer.
- Microorganisms can be made skillful to take up a plasmid by treating it with a particular convergence of divalent cation, for example, calcium, which makes the DNA progressively productive to enter the pores of the cell divider.
- To bring the outsider DNA into the plant have cell vector is utilized, for example, Plasmid (extra chromosomal DNA of microorganisms). The outside DNA is consolidated into the plasmid by the methods for limitation compound, at that point the remote DNA is joined by the DNA ligase protein which is then fused.
- Disarmed vectors are the vectors, which when taint the cell moves the recombinant DNA into the host. Agro bacterium tumefaciens is one such disarmed vector.
Similar questions