why DNA is negatively charged??????
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The phosphate backbone of DNA is negatively charged due to the bonds created between the phosphorous atoms and the oxygen atoms. Each phosphate group contains one negatively charged oxygen atom, therefore the entire strand of DNA is negatively charged due to repeated phosphate groups.
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The reason why DNA is negatively charged is the phosphate group that makes up every nucleotide (pentose + nitrogenous base + phosphate). When forming part of the phosphodiester bond, they retain 1 of 2 negative charges (the other being lost to form the other ester bond to a new pentose, that's why the bond is called "phospho-di-ester").
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