Question

Why do cytoplasm absorb less stain?

Answer 1

Cytoplasm tends to be much poorer in acidic materials than nuclei (there are some expect ions to this statement ) and the cytoplasmic reaction with hematoxylin (or other basic stains) tends to be markedly less than that of the nucleus . Frequently,cytoplasm is termed acidophiles or (in the case of HE slides) eosinophilic in its staining reaction