Question

Why do proteins precipitate at their isoelectric point?

Answer 1

Explanation:

the surface of the protein is predominantly negatively charged and therefore like-charged molecules will exhibit repulsive forces. ... For this reason isoelectric point precipitation is most often used to precipitate contaminant proteins, rather than the target protein.

Answer 2

Answer:

At a solution pH that is above the pI the surface of the protein is predominantly negatively charged and therefore like-charged molecules will exhibit repulsive forces. ... For this reason isoelectric point precipitation is most often used to precipitate contaminant proteins, rather than the target protein.

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