Question

why do some of the transfected bacteria grow on plates containing ampicillin?​

Answer 1

Answer:

coli cells are affected by ampicillin. Equal amounts of E. coli cells could be plated on two different LB nutrient agar plates, one which contains just LB nutrient agar and one which contains LB nutrient agar ampicillin. The growth of the E.

Answer 2

Answer:

They typically appear because beta-lactamase secreted by the transformed vector cells degrade the antibiotics around the transformed cells. Thus leading to growth of non-transformed colonies. Satellite colonies also appear when pH of medium is below 7 as beta-lactamase is inactive below pH7 and other thing is bacterial growth makes media pH acidic a bit. We usually keep pH of ampicillin media 7.4 and avoid overgrowth of cells.

Explanation:

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