Question

Why do we add dideoxynucleotides in sangers sequencing method?

Answer 1

Dideoxynucleotides are chain-elongating inhibitors of DNApolymerase, used in the Sanger method for DNA sequencing. They arealso known as 2',3'dideoxynucleotides, and abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP). ... Dideoxynucleotides areuseful in the sequencing of DNA in combination with electrophoresis