Why do we add guanidium thiocynate in rna isloatiom?
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Guanidine thiocyanate is an extremely potent chaotropic agent; thus, it quickly denatures macromolecules and inactivates nucleases. Phenol extractions are then done at 60°C, thereby preferentially extracting RNA into the aqueous phase and leaving the DNA behind.
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Guanidine thiocyanate is an extremely potent chaotropic agent; thus, it quickly denatures macromolecules and inactivates nucleases. Phenol extractions are then done at 60°C, thereby preferentially extracting RNA into the aqueous phase and leaving the DNA behind.
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