Why do we boil a leaf in water before testing it with iodine.
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This procedure kills a leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present.
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It is very important to dip the leaves in boiling water before adding the iodine solution while conducting the starch test.
This helps to get rid of the waxy cuticle that blocks the entry of iodine, damages cell membranes to form starch granules in cytoplasm and chloroplasts.
It also alters the natural quality of those enzyme that convert starch to glucose
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