why do we keep the conical flask airtight
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we keep the conical flask airtight because that the KOH solution didn't absorb the CO2 of upper half of leaf.
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KOH solution kept in the test-tube inside the air-tight conical flask absorbs the evolved carbon dioxide released by germinating seeds; thereby creating a partial vacuum in the conical flask. So, an equal volume of water rises up in the tube. This indicates that the germinating seeds are actively respiring and CO2 gas is evolving in the process of germination.
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