Why do we put the green leaf in water to boil?
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This procedure kills a leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present.
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ᴛʜɪs ᴘʀᴏᴄᴇᴅᴜʀᴇ ᴋɪʟʟs ᴀ ʟᴇᴀғ, ᴅɪsʀᴜᴘᴛs ᴛʜᴇ ᴄᴇʟʟ ᴍᴇᴍʙʀᴀɴᴇs ᴀɴᴅ sᴏғᴛᴇɴs ᴛʜᴇ ᴄᴜᴛɪᴄʟᴇ ᴀɴᴅ ᴄᴇʟʟ ᴡᴀʟʟs. ᴛʜɪs ᴍᴀᴋᴇs ɪᴛ ᴘᴏssɪʙʟᴇ ᴛᴏ ᴇxᴛʀᴀᴄᴛ ᴛʜᴇ ᴄʜʟᴏʀᴏᴘʜʏʟʟ ᴡɪᴛʜ ʜᴏᴛ ᴇᴛʜᴀɴᴏʟ ᴀɴᴅ ᴀʟsᴏ ᴀʟʟᴏᴡs ᴛʜᴇ ɪᴏᴅɪɴᴇ sᴏʟᴜᴛɪᴏɴ ᴛᴏ ᴘᴇɴᴇᴛʀᴀᴛᴇ ᴛʜᴇ ᴄᴇʟʟs ᴀɴᴅ ʀᴇᴀᴄᴛ ᴡɪᴛʜ ᴀɴʏ sᴛᴀʀᴄʜ ᴘʀᴇsᴇɴᴛ.
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