Why do we use a negative control in PCR?
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Negative controls should be analyzed to verify that no contaminating nucleic acid has been introduced into the master mix or into samples during sample processing. ... The PCR positive and negative controls can be used as the positive and negative gel electrophoresis controls, respectively.
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Explanation:
Negative control is used to test that your PCR reaction is specific and amplifies only what you want. If you get results in your negative control test tube, it means that something is amiss and your reaction is not specific. Accordingly, the results of such PCR session won’t be useful in any way, because they are likely to contain impurities.
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