Why do we use iodine
solution to destarch the leaf
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The alcohol removes the chlorophyll that makes the leaves green and the heat softens the leaf's cell walls making chemical reactions easier to do . Dip this leaf into a dilute solution of iodine and watch the leaf turn dark green as a result of starch and iodine combining to form a violet coloured complex.
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Iodine is used as a test for starch (both linear Amylose and branched Amylopectin) because of the distinct colour change, deep orange to dark blue, that occurs when it forms a polyiodide complex with certain polysaccharides. Please note that it is not a test for Cellulose due to the different structure of this polysaccharide.
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