Why do we use safranin as a strain???
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Then we have given that 5 times this angle is equal to its supplement that is 180∘.
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Because cells typically have negatively charged cell walls, the positive chromophores in basic dyes tend to stick to the cell walls, making them positive stains. Thus, commonly used basic dyes such as basic fuchsin, crystal violet, malachite green, methylene blue, and safranin typically serve as positive stains.
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