Question

Why do you heat the upper part of the tube in heat and acetic acid test?

Answer 1

Answer:

This is your answer :

Explanation:

Heat coagulation test is used to detect the presence of albumin and globulin protein in the urine sample. As albumin and globulin in urine are observed under many pathological conditions, their presence can be conclusively established by this test, which aids in the diagnosis of diseases.

Answer 2

The upper part of the tube in heat and acetic acid test is heated to test the presence of proteins in the urine sample.

Explanation:

• Heat and acetic acid test is used for the detection of proteins in the given urine sample.
• The test is based on the principle that proteins get coagulated when they are boiled in an acidic medium.
• In this test, about 5-10 ml of urine is taken in a test tube.
• Then, the upper portion of the test tube is heated and the upper and lower portions are compared.
• If turbidity occurs, it indicates the presence of either phosphates/carbonates or proteins.
• Now, 2-4 drops of $\[10\% \,\]$ Glacial acetic acid are added to the test tube and the upper portion is boiled again.
• If the turbidity disappears, then the sample contains phosphates/carbonates but if the turbidity is still present, then the sample contains proteins.

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