Question

Why does a f- recipient cell remains f- even after conjugation with an hfr donor, but remains f+ after mating with f+?

Answer 1

$\huge\mathfrak{Answer:}$

During conjugation between an Hfr donor and an F− recipient, the genes of the donor are transmitted linearly to the F− cell, through the bacterial chromosome, with the inserted fertility factor transferring last. The transmitted part was originally adjacent to the F locus in an Hfr strain from which the F+ was derived.

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