Why does preparation of lb require autoclave?
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On solid media, a single microbe will grow and divide to produce a "colony," a spot of identical descendants. Different types ... Decide how many plates you will need. ... Prepare ...
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▶This is an important step because dry culture media powder above the level of the water may not be sterilized in the autoclave and may be a source of contamination. Agar-free media will usually dissolve on gentle agitation. Media containing agar should be heated to dissolve the agar before autoclaving
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